Activation of TLR3 in keratinocytes increases expression of genes involved in formation of the epidermis, lipid accumulation, and epidermal organelles.

Injury to the skin, and the subsequent release of noncoding double-stranded RNA (dsRNA) from necrotic keratinocytes, has been identified as an endogenous activator of Toll-like receptor 3 (TLR3). As changes in keratinocyte growth and differentiation follow injury, we hypothesized that TLR3 might trigger some elements of the barrier repair program in keratinocytes. dsRNA was observed to induce TLR3-dependent increases in human keratinocyte mRNA abundance for ABCA12 (ATP-binding cassette, sub-family A, member 12), glucocerebrosidase, acid sphingomyelinase, and transglutaminase 1. Additionally, treatment with dsRNA resulted in increases in sphingomyelin and morphologic changes including increased epidermal lipid staining by Oil Red O and TLR3-dependent increases in lamellar bodies and keratohyalin granules. These observations show that dsRNA can stimulate some events in keratinocytes that are important for skin barrier repair and maintenance

Nutritional, Bone, and Physical Fitness States of Jockeys in Chronic Repetition of Short-Term Weight Loss

Professional jockeys are required to fit their weight, mainly weight loss, for two game days every weekend during racing season which usually lasts 10 months a year in Korea. The repetitive routine weight loss protocol is performed for 4 to 5 days a week include two game days. This situation would have brought some negative effects on the professional jockeys\u27 overall health and physical condition with the relation of their performance. The purpose of the present study was: 1) to examine the weight loss habits of pro-jockeys, and 2) effects of chronic short-term weight loss on nutritional status, bone health, and physical fitness of them. Pro jockeys (n=43) completed a questionnaire for the assessment of weight loss habits. Pro jockeys (n=10, around 12 years of career) were compared with trainee jockeys (n=10, less than 1 years of career) for their nutritional status, bone health, and physical fitness. The nutritional status was measured by a computerized program (CAN Pro 2.0). Bone mineral density was measured by DEXA (Hologic, QDR-4500W, USA). All participants performed grip strength test, standing on one leg with eyes closed test, and side step test for the assessment of physical fitness. According to the result, more than 80% of pro jockeys underwent a routine weight loss process every week, for around 1 kg, within 2 to 3 days prior to the game days by diet mainly and/or exercise/sauna. Around 70% of them reported severe fatigue during the weight loss period. There were no significant differences in nutritional status between two groups. However, they consume average of 1,100 Kcal a day reflecting 21 to 80% of recommended daily consumption during weight loss periods. In bone health, six trainee jockeys and two pro jockeys were diagnosed as osteopenia. One pro jockey was diagnosed as osteoporosis. No significant difference was found in fitness factors between two groups. In conclusion, chronic exposure to short-term weight loss may result in serious health problems especially on bone health. For the jockeys in Korea, balanced diet program and the related education on weight management are strongly required.

Improvement of Saemangeum Dredged Soils Using Coffee Sludge for Vegetation Soil

In Korea, a large scale national project (Saemangeum Project) has been underway that requires a huge amount of dredged soils and their reclamation. Although a lot of dredged soil is needed for reclamation, only about 10% of the dredged soil is used. For this reason, much effort should be made to extensively use the dredged soil. The objective of the study is to find reasonable ways of improving the dredged soils in the Saemangeum area so that they can be used for vegetation of land plants. In order to develop ameliorating methods, we treated silty sand samples, the representative dredged soil of Saemangeum, with mountain soil (0% and 30%), sawdust fertilizer (0% and 6%), bioameliorant (0% and 6%), and coffee sludge (3%, 6%, and 9%), measured the germination rate of bent grass, and applied the lab experiment results to the field for validation. As a result, it was verified that when a mixture of coffee sludge and sawdust fertilizer was used, the chemical and physical properties of dredged soil were significantly improved. This implies that the beneficial use of the dredged soil can be facilitated

A Study of Double Dark Photons Produced by Lepton Colliders using High Performance Computing

The universe is thought to be filled with not only Standard Model (SM) matters but also dark matters. Dark matter is thought to play a major role in its construction. However, the identity of dark matter is as yet unknown, with various search methods from astrophysical observartion to particle collider experiments. Because of the cross-section that is a thousand times smaller than SM particles, dark matter research requires a large amount of data processing. Therefore, optimization and parallelization in High Performance Computing is required. Dark matter in hypothetical hidden sector is though to be connected to dark photons which carries forces similar to photons in electromagnetism. In the recent analysis, it was studied using the decays of a dark photon at collider experiments. Based on this, we studies double dark photon decays at lepton colliders. The signal channels are e+e– → AʹAʹ and e+e– → AʹAʹγ where dark photon Aʹ decays dimuon. These signal channels are based on the theory that dark photons only decay into heavily charged leptons, which can explain the muon magnetic momentum anomaly. We scanned the cross-section according to the dark photon mass in experiments. MadGraph5 was used to generate events based on a simplified model. Additionally, to get the maximum expected number of events for the double dark photon channel, the detector efficiency for several center of mass (CM) energy were studied using Delphes and MadAnalysis5 for performance comparison. The results of this study will contribute to the search for double dark photon channels at lepton colliders

A Cyber Command and Control Framework for Psychological Operation Using Social Media

Global threats, international terrorist groups and North Korea, paralyze political decisions by attacking and neutralizing the credibility of the main policy makers in the state and simultaneously manipulate the public opinion, which results in distrust and disconnection between each other. These threats use social media as their biggest core routine to conduct such attacks. This paper presents a series of processes and frameworks on how a commander should make a decision when performing a cyber psychological operation using social media. Based on the Endsley model, which is a situational awareness model, the paper compares the strengths and weaknesses of the three social media operations (IGMO, DeSMO, OSMO) performed by the military and proposes a guideline for performing an operation

Exosomes from Human Adipose Tissue-Derived Mesenchymal Stem Cells Promote Epidermal Barrier Repair by Inducing de Novo Synthesis of Ceramides in Atopic Dermatitis.

Atopic dermatitis (AD) is a multifactorial, heterogeneous disease associated with epidermal barrier disruption and intense systemic inflammation. Previously, we showed that exosomes derived from human adipose tissue-derived mesenchymal stem cells (ASC-exosomes) attenuate AD-like symptoms by reducing multiple inflammatory cytokine levels. Here, we investigated ASC-exosomes' effects on skin barrier restoration by analyzing protein and lipid contents. We found that subcutaneous injection of ASC-exosomes in an oxazolone-induced dermatitis model remarkably reduced trans-epidermal water loss, while enhancing stratum corneum (SC) hydration and markedly decreasing the levels of inflammatory cytokines such as IL-4, IL-5, IL-13, TNF-α, IFN-γ, IL-17, and TSLP, all in a dose-dependent manner. Interestingly, ASC-exosomes induced the production of ceramides and dihydroceramides. Electron microscopic analysis revealed enhanced epidermal lamellar bodies and formation of lamellar layer at the interface of the SC and stratum granulosum with ASC-exosomes treatment. Deep RNA sequencing analysis of skin lesions demonstrated that ASC-exosomes restores the expression of genes involved in skin barrier, lipid metabolism, cell cycle, and inflammatory response in the diseased area. Collectively, our results suggest that ASC-exosomes effectively restore epidermal barrier functions in AD by facilitating the de novo synthesis of ceramides, resulting in a promising cell-free therapeutic option for treating AD